Equipment list

Vasilievsky island analytical and instrumental site

Transmission electron microscopy (TEM)

  • Jeol JEM-1400 Transmission Electron Microscope
    • Side-mounted TEM CCD camera Olympus Veleta
  • Jeol JEM-2100HC Transmission Electron Microscope
    • Side-mounted TEM CCD camera Gatan ES500W Erlangshen (782)
    • Bottom mounted TEM CCD camera Gatan Ultrascan 4000 (895)

Complementary TEM equipment

  • Jeol JEE-420D Vacuum Evaporator
  • Support Film Hydrophilizing System Jeol HDT-400
  • Imaging Plate Documentation System DitaBis MicronVario
  • Ion Cleaning Device Jeol JIC-410
  • Jeol EM-DSC30 Photoplate degasser
  • Gatan 655 Turbo Pumping Station
  • Gatan 914 Nitrogen Cooled Cryotransfer Tomography Holder with Gatan 900 temperature controller

Scanning electron microscopy (SEM)

  • Benchtop Scanning Electron Microscope Jeol JCM-5000 NeoScope
  • Tescan MIRA3 LMU Scanning Electron Microscope + Leica EM VCT100 contamination-free cryo transfer system + Oxford X-Max 80 energy-dispersive X-ray spectroscopy system

Electron microscopy sample preparation

  • Ultramicrotome Leica EM UC7
  • Ultramicrotome for Sectioning at Room Temperature and Cryo Leica EM UC7 with Cryochamber Leica EM FC7
  • Glass Knifemaker Leica EM KMR3
  • High vacuum sputter coater for highest resolution FE-SEM analysis Leica EM SCD500
  • Freeze Fracture System Leica EM BAF060 with Vacuum cryo transfer system Leica EM VCT100
  • Automated Critical Point Dryer Leica EM CPD300
  • Freeze Substitution and Low Temperature Embedding System Leica EM AFS2
  • Automatic Plunge Freezing with Bare Grid Technique Leica EM GP
  • Specimen trimming device Leica EM TRIM2
  • Automated immunogold labeling system Leica EM IGL
  • Automatic Microwave Tissue Processor for Electron Microscopy Leica EM AMW
  • Automatic contrasting instrument for ultrathin sections Leica EM AC20
  • High Pressure Freezer for Cryofixation of Biological And Industrial Samples Leica EM HPM100

Light microscopy

  • Scanning confocal microscope with multi-photon excitation Leica TCS SP5 MP
    • Leica TCS STED CW Super-resolution System
    • Fluorescence Lifetime Correlation Spectroscopy System Leica TCS SMD FLCS
    • Leica Pulsed White Light Laser
  • Scanning confocal microscope Leica TCS SP5
  • Spinning Disk Confocal Microscope Zeiss Cell Observer SD
  • Automated Fluorescence Stereo Microscope Leica M205 FA
  • Fully Automated Inverted Fluorescence Microscope for Biomedical Research Leica DMI6000 B illumination system OptiGrid
  • Leica LMD7000 Laser Microdissection System
  • Routine Stereo Microscopes Leica M80 and Leica M125
  • Automated Upright Microscope for Life Science Research Leica DMI4000 B
  • Inverted Laboratory Microscope with LED Illumination Leica DM IL LED
  • SVI 3D Huygens Deconvolution & Analysis Software Package
  • Bitplane Imaris Software Package

Light microscopy sample preparation

  • Leica Vibratome VT-1200S
  • Fully Automated Rotary Microtome Leica RM2265
  • Manual Rotary Microtome for Routine Sectioning Leica RM2235 with CoolClamp
  • Sliding microtome Leica SM2010 R
  • Research Cryostat Leica CM3050 S
  • Modular Tissue Embedding System Leica EG1150 H, EG1150 C, EG F
  • Multistainer Leica ST5020
  • Fully Automated Glass Coverslipper Leica CV5030
  • Automated routine tissue processor Leica EM TP-1020VF 
  • Multifunctional Microwave Tissue Processor Milestone KOS
  • Dissection Table Kugel UCS-E-1750-D
  • Liquid robotics system Tecan GenePaint Evo 150/4

Cell cultures equipment

  • BD (Becton, Dickinson and Company) FACSAria III Cell Sorter
  • Bio-Rad Gene Pulser Xcell Electroporation System
  • Micropipettes and microelectrodes preparation systems:
    • Sutter Instruments P-1000 Micropipette Puller
    • Narishige MF-900 Microforge
    • Sutter Instruments BV-10 Microelectrode Beveler
  • BioRad TC10 Automated Cell Counter
  • Eppendorf Brunswick Galaxy CO170R CO2 Incubator
  • Eppendorf Brunswick Galaxy CO14S CO2 Incubator
  • SafeFASТ Elite 218S Class II Microbiological Safety Cabinet
  • Cryostorage system Taylor-Wharton 10K
  • Routine Equipment:
    • Stirred Water Bath BioSan BWT-U
    • Horizontal autoclave for liquid and instrument sterilization Tuttnauer 2540EKA
    • Vertical Automatic Autoclave with cooling system Tuttnauer 3870ELVС
    • Nalgene S-C1562 Freezing Container
    • Sigma Bright-Line Hemocytometer
Cell culture equipment is scheduled for installation in december 2013.

Proteomics, metabolomics, genomics equipment

  • Agilent 6538 UHD Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) Liquid Chromatograph Mass Spectrometer (LC/MS)
  • Agilent 1260 High-Performance Liquid Chromatograch (HPLC) on board of the Agilent 6538 UHD System
  • High-Throughput Gas Chromatography with Time-of-Flight Mass Spectrometer LECO Pegasus 4D GCxGC-TOFMS
  • The Agilent 7890 Two-Dimensional (2D) GCxGC Gas Chromatograph on board of the LECO Pegasus 4D GCxGC-TOFMS
  • High-performance Field and Laboratory Chlorophyll Fluorometer Walz PAM-2500
  • Bio-Rad CHEF MAPPER XA Pulsed Electrophoresis System
  • Agilent Cary Eclipse Fluorescence Spectrophotometer
  • NanoDrop 2000 Spectrophotometer
  • Beckman Coulter Optima MAX-XP Ultracentrifuge
  • Labconco CentriVap Centrifugal Vacuum Concentrator
  • Agilent 2100 Bioanalyzer
  • Protein electrophoresis and blotting systems uncluding BioRad Isoelectric Focusing (IEF) System
  • Typhoon FLA 9500 Laser Scanner
  • BioRad GS-800 Calibrated Densitomete
  • BioRad ChemiDoc MP Imaging System
  • BioRad Prep Cell Preparative Electrophoresis System
  • BioRad Rotofor Isoelectrofocusing System
  • Bio-Rad C1000 Thermal Cycler
  • Bio-Rad CFX96 Real-Time PCR Detection System
  • Retsch MM 400 Mixer Mill
  • Heidolph SilentCrusher S Homogenizer of sample quiantities 0.8-10 ml
  • Bench Top PCR Box "Laminar Systems" BAV-PCR-Laminar-S
  • GFL Hybridisation Incubator 7601
  • UVP CL-1000 Ultraviolet Crosslinker
  • Incubating Shaker GFL 3033 with Analogue Orbital Shaker GFL 3015
  • Merck Millipore SNAP i.d. 2.0 Protein Detection System
  • Routine laboratory equipment (balances, centrifuges, vortex, pipettes, etc.):
    • Eppendorf Centrifuges 5810R, 5415R, 5702, MiniSpin Plus
    • Distillator GFL-2008
    • Invitrogen Qubit 2.0 Fluorometer
    • Precision Top Loading Balance Ohaus Adventurer Pro OH-AV413C
    • Analytical Balance CPA-324S
    • Sartorius pH Meter PB-11
    • Personal voltex BioSan V-1 plus
    • Magnetic Stirrer with hot plate BioSan MSH-300
    • Gravity Convection Incubator Binder BD53 and Binder BD23
    • Plate Shaker-Thermostat BioSan PST-60 HL
    • Solid-state Shaker-Thermostat Eppendorf Thermomixer Comfort
    • Mini-Rocker Shaker BioSan MR-1
    • Shaker BioSan Multi Bio 3D
    • Multi Speed Vortex BioSan MSV-3500 with additional platforms MSV-SV-16/8 and SV-8/15
    • Programmable rotator BioSan Multi Bio RS-24/PRS-22
    • Solid-state Thermostat DNA-technology "Gnom"
    • Analitical Quality Water (18 Megaohm) Purification System Mediana-Filter UVOI-"MF"-1NA(18)-2
    • Skadi IF90 Ice Flake Machine
    • 8-channel pipettes HTL-DV8-10, HTL-DV8-50, HTL-DV8-300
    • One-channel pipettes of different volumes 0,1- 2,5 µl, 0.5-10 µl, 2-20 µl, 20-200 µl, 100-1000 µl
    • Eppendorf Multipette Xstream electronic hand dispenser
    • Elma Elmasonic S100 H Ultrasonic Bath
Equipment for DNA, RNA, protein and different metabolite isolation is scheduled for installation in novermber 2013.

Petergof analytical site


  • Protein electrophoresis and blotting systems uncluding BioRad Isoelectric Focusing (IEF) System
  • BioRad ChemiDoc MP Imaging System
  • BioRad xMark Microplate Absorbance Spectrophotometer
  • NanoDrop 2000 Spectrophotometer
  • BioRad EXQuest Spot Cutter with BioRad PDQuest 2-D Analysis Software
  • Bio-Rad ProteOn XPR36 Protein Interaction Array System
  • Waters Breeze 2 High Performance Liquid Chromatograph
  • Thermo Scientific Dionex UltiMate 3000 NanoFlow Liquid Chromatograph with Bruker Proteineer FC II LC-MALDI Fractions Collector
  • Matrix Deposition Device for MALDI Imaging in Histology Bruker ImagePrep
  • Bruker UltrafleXtreme MALDI-TOT/TOF Mass Spectrometer
  • Beckman Coulter Avanti J-30I Centrifuge
  • Beckman Coulter Optima L-100XP Preparative Ultracentrifuge


  • Roche 454 GS Junior Sequencing System
  • ABI Ion Torrent PGM Sequencer
  • ABI PRISM 310 Genetic Analyzer
  • ABI PRISM 3500xL Genetic Analyzer
  • Nucleic acid Electrophoresis Aystem
  • Sage Pippin Prep DNA size selection system
  • Diagenode BioRuptor UCD-200 DNA Fragmentation System
  • ABI Ion One Touch Robotics System for ABI Ion Torrent PGM Sequencer
  • Microchip Electrophoresis System for DNA/RNA Analysis Shimadzu MCE-202 MultiNA
  • Bio-Rad CFX96 Real-Time PCR Detection System
  • Microfluidic PCR device Fluidigm BioMark HD
  • Bio-Rad QX-100 Droplet Digital PCR System
  • Hamilton REM e STARlet Liquid Handeling Workstation
  • DNAStar Lasergene 10 Software

Complementary equipment

  • MPbiomed FastPrep-24 Homogenizer
  • Bio-Rad Gene Pulser Xcell Electroporation System
  • Routine Equipment (centrifuges, shakers, laminar boxes, PCR-boxes, autoclaves, water purification systems)

See also: