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Equipment list
Vasilievsky island analytical and instrumental site
Transmission electron microscopy (TEM)
- Jeol JEM-1400 Transmission Electron Microscope
- Side-mounted TEM CCD camera Olympus Veleta
- Jeol JEM-2100HC Transmission Electron Microscope
- Side-mounted TEM CCD camera Gatan ES500W Erlangshen (782)
- Bottom mounted TEM CCD camera Gatan Ultrascan 4000 (895)
Complementary TEM equipment
- Jeol JEE-420D Vacuum Evaporator
- Support Film Hydrophilizing System Jeol HDT-400
- Imaging Plate Documentation System DitaBis MicronVario
- Ion Cleaning Device Jeol JIC-410
- Jeol EM-DSC30 Photoplate degasser
- Gatan 655 Turbo Pumping Station
- Gatan 914 Nitrogen Cooled Cryotransfer Tomography Holder with Gatan 900 temperature controller
Scanning electron microscopy (SEM)
- Benchtop Scanning Electron Microscope Jeol JCM-5000 NeoScope
- Tescan MIRA3 LMU Scanning Electron Microscope + Leica EM VCT100 contamination-free cryo transfer system + Oxford X-Max 80 energy-dispersive X-ray spectroscopy system
Electron microscopy sample preparation
- Ultramicrotome Leica EM UC7
- Ultramicrotome for Sectioning at Room Temperature and Cryo Leica EM UC7 with Cryochamber Leica EM FC7
- Glass Knifemaker Leica EM KMR3
- High vacuum sputter coater for highest resolution FE-SEM analysis Leica EM SCD500
- Freeze Fracture System Leica EM BAF060 with Vacuum cryo transfer system Leica EM VCT100
- Automated Critical Point Dryer Leica EM CPD300
- Freeze Substitution and Low Temperature Embedding System Leica EM AFS2
- Automatic Plunge Freezing with Bare Grid Technique Leica EM GP
- Specimen trimming device Leica EM TRIM2
- Automated immunogold labeling system Leica EM IGL
- Automatic Microwave Tissue Processor for Electron Microscopy Leica EM AMW
- Automatic contrasting instrument for ultrathin sections Leica EM AC20
- High Pressure Freezer for Cryofixation of Biological And Industrial Samples Leica EM HPM100
Light microscopy
- Scanning confocal microscope with multi-photon excitation Leica TCS SP5 MP
- Leica TCS STED CW Super-resolution System
- Fluorescence Lifetime Correlation Spectroscopy System Leica TCS SMD FLCS
- Leica Pulsed White Light Laser
- Scanning confocal microscope Leica TCS SP5
- Spinning Disk Confocal Microscope Zeiss Cell Observer SD
- Automated Fluorescence Stereo Microscope Leica M205 FA
- Fully Automated Inverted Fluorescence Microscope for Biomedical Research Leica DMI6000 B illumination system OptiGrid
- Leica LMD7000 Laser Microdissection System
- Routine Stereo Microscopes Leica M80 and Leica M125
- Automated Upright Microscope for Life Science Research Leica DMI4000 B
- Inverted Laboratory Microscope with LED Illumination Leica DM IL LED
- SVI 3D Huygens Deconvolution & Analysis Software Package
- Bitplane Imaris Software Package
Light microscopy sample preparation
- Leica Vibratome VT-1200S
- Fully Automated Rotary Microtome Leica RM2265
- Manual Rotary Microtome for Routine Sectioning Leica RM2235 with CoolClamp
- Sliding microtome Leica SM2010 R
- Research Cryostat Leica CM3050 S
- Modular Tissue Embedding System Leica EG1150 H, EG1150 C, EG F
- Multistainer Leica ST5020
- Fully Automated Glass Coverslipper Leica CV5030
- Automated routine tissue processor Leica EM TP-1020VF
- Multifunctional Microwave Tissue Processor Milestone KOS
- Dissection Table Kugel UCS-E-1750-D
- Liquid robotics system Tecan GenePaint Evo 150/4
Cell cultures equipment
- BD (Becton, Dickinson and Company) FACSAria III Cell Sorter
- Bio-Rad Gene Pulser Xcell Electroporation System
- Micropipettes and microelectrodes preparation systems:
- Sutter Instruments P-1000 Micropipette Puller
- Narishige MF-900 Microforge
- Sutter Instruments BV-10 Microelectrode Beveler
- BioRad TC10 Automated Cell Counter
- Eppendorf Brunswick Galaxy CO170R CO2 Incubator
- Eppendorf Brunswick Galaxy CO14S CO2 Incubator
- SafeFASТ Elite 218S Class II Microbiological Safety Cabinet
- Cryostorage system Taylor-Wharton 10K
- Routine Equipment:
- Stirred Water Bath BioSan BWT-U
- Horizontal autoclave for liquid and instrument sterilization Tuttnauer 2540EKA
- Vertical Automatic Autoclave with cooling system Tuttnauer 3870ELVС
- Nalgene S-C1562 Freezing Container
- Sigma Bright-Line Hemocytometer
Cell culture equipment is scheduled for installation in december 2013.
Proteomics, metabolomics, genomics equipment
- Agilent 6538 UHD Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) Liquid Chromatograph Mass Spectrometer (LC/MS)
- Agilent 1260 High-Performance Liquid Chromatograch (HPLC) on board of the Agilent 6538 UHD System
- High-Throughput Gas Chromatography with Time-of-Flight Mass Spectrometer LECO Pegasus 4D GCxGC-TOFMS
- The Agilent 7890 Two-Dimensional (2D) GCxGC Gas Chromatograph on board of the LECO Pegasus 4D GCxGC-TOFMS
- High-performance Field and Laboratory Chlorophyll Fluorometer Walz PAM-2500
- Bio-Rad CHEF MAPPER XA Pulsed Electrophoresis System
- Agilent Cary Eclipse Fluorescence Spectrophotometer
- NanoDrop 2000 Spectrophotometer
- Beckman Coulter Optima MAX-XP Ultracentrifuge
- Labconco CentriVap Centrifugal Vacuum Concentrator
- Agilent 2100 Bioanalyzer
- Protein electrophoresis and blotting systems uncluding BioRad Isoelectric Focusing (IEF) System
- Typhoon FLA 9500 Laser Scanner
- BioRad GS-800 Calibrated Densitomete
- BioRad ChemiDoc MP Imaging System
- BioRad Prep Cell Preparative Electrophoresis System
- BioRad Rotofor Isoelectrofocusing System
- Bio-Rad C1000 Thermal Cycler
- Bio-Rad CFX96 Real-Time PCR Detection System
- Retsch MM 400 Mixer Mill
- Heidolph SilentCrusher S Homogenizer of sample quiantities 0.8-10 ml
- Bench Top PCR Box "Laminar Systems" BAV-PCR-Laminar-S
- GFL Hybridisation Incubator 7601
- UVP CL-1000 Ultraviolet Crosslinker
- Incubating Shaker GFL 3033 with Analogue Orbital Shaker GFL 3015
- Merck Millipore SNAP i.d. 2.0 Protein Detection System
- Routine laboratory equipment (balances, centrifuges, vortex, pipettes, etc.):
- Eppendorf Centrifuges 5810R, 5415R, 5702, MiniSpin Plus
- Distillator GFL-2008
- Invitrogen Qubit 2.0 Fluorometer
- Precision Top Loading Balance Ohaus Adventurer Pro OH-AV413C
- Analytical Balance CPA-324S
- Sartorius pH Meter PB-11
- Personal voltex BioSan V-1 plus
- Magnetic Stirrer with hot plate BioSan MSH-300
- Gravity Convection Incubator Binder BD53 and Binder BD23
- Plate Shaker-Thermostat BioSan PST-60 HL
- Solid-state Shaker-Thermostat Eppendorf Thermomixer Comfort
- Mini-Rocker Shaker BioSan MR-1
- Shaker BioSan Multi Bio 3D
- Multi Speed Vortex BioSan MSV-3500 with additional platforms MSV-SV-16/8 and SV-8/15
- Programmable rotator BioSan Multi Bio RS-24/PRS-22
- Solid-state Thermostat DNA-technology "Gnom"
- Analitical Quality Water (18 Megaohm) Purification System Mediana-Filter UVOI-"MF"-1NA(18)-2
- Skadi IF90 Ice Flake Machine
- 8-channel pipettes HTL-DV8-10, HTL-DV8-50, HTL-DV8-300
- One-channel pipettes of different volumes 0,1- 2,5 µl, 0.5-10 µl, 2-20 µl, 20-200 µl, 100-1000 µl
- Eppendorf Multipette Xstream electronic hand dispenser
- Elma Elmasonic S100 H Ultrasonic Bath
Equipment for DNA, RNA, protein and different metabolite isolation is scheduled for installation in novermber 2013.
Petergof analytical site
Proteomics
- Protein electrophoresis and blotting systems uncluding BioRad Isoelectric Focusing (IEF) System
- BioRad ChemiDoc MP Imaging System
- BioRad xMark Microplate Absorbance Spectrophotometer
- NanoDrop 2000 Spectrophotometer
- BioRad EXQuest Spot Cutter with BioRad PDQuest 2-D Analysis Software
- Bio-Rad ProteOn XPR36 Protein Interaction Array System
- Waters Breeze 2 High Performance Liquid Chromatograph
- Thermo Scientific Dionex UltiMate 3000 NanoFlow Liquid Chromatograph with Bruker Proteineer FC II LC-MALDI Fractions Collector
- Matrix Deposition Device for MALDI Imaging in Histology Bruker ImagePrep
- Bruker UltrafleXtreme MALDI-TOT/TOF Mass Spectrometer
- Beckman Coulter Avanti J-30I Centrifuge
- Beckman Coulter Optima L-100XP Preparative Ultracentrifuge
Genomics
- Roche 454 GS Junior Sequencing System
- ABI Ion Torrent PGM Sequencer
- ABI PRISM 310 Genetic Analyzer
- ABI PRISM 3500xL Genetic Analyzer
- Nucleic acid Electrophoresis Aystem
- Sage Pippin Prep DNA size selection system
- Diagenode BioRuptor UCD-200 DNA Fragmentation System
- ABI Ion One Touch Robotics System for ABI Ion Torrent PGM Sequencer
- Microchip Electrophoresis System for DNA/RNA Analysis Shimadzu MCE-202 MultiNA
- Bio-Rad CFX96 Real-Time PCR Detection System
- Microfluidic PCR device Fluidigm BioMark HD
- Bio-Rad QX-100 Droplet Digital PCR System
- Hamilton REM e STARlet Liquid Handeling Workstation
- DNAStar Lasergene 10 Software
Complementary equipment
- MPbiomed FastPrep-24 Homogenizer
- Bio-Rad Gene Pulser Xcell Electroporation System
- Routine Equipment (centrifuges, shakers, laminar boxes, PCR-boxes, autoclaves, water purification systems)
See also:
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